PrimerServer

PrimerServer v2.0.0b21 PCR Primers Batch Design & Specificity Check

Input: Design primers and check specificity

We recommend to run the CLI version to generate the JSON output, and visualize the results in the [ Visualize] tab above this website.

Selected Templates

Parameter: Design

Region Type

Product Size (bp)

Min Max

No. Primers To Detect

Parameter: Check Specificity

Checking Amplicon Size (bp)

Min Max

Min. Tm Difference:

(°C) lower

Filter with 3' End Validation:

Yes

Get Amplicon Seq:

Yes

Parameter: General

Max. Primers Return Number:

Check Dimers(For multiplex PCR):

Yes

Site 1	Primer 1	Seq 1	Site 2	Primer 2	Seq 2	Tm (°C)	Structure

PrimerServer v2.0.0b21 PCR Primers Batch Design & Specificity Check

Possible Amplicons